NST PMS 1B: Introduction

Prof. Jim Haseloff (jh295@cam.ac.uk)
Links to supplementary lecture materials below:
Lecture 1. Plant breeding and transformation
(Click to see slide show)
(i) Crop breeding: Zea mays as an example, hybrid maize and the rise of agribusiness
(ii) "Ground Zero" for biotechnology
Agrobacterium mediated plant transformation
First plant transformation experiments
Biotechnology in agriculture
(Download Lecture 1 notes in PDF form)
Suggested reading
Biotechnology in the 1930s: the development of hybrid maize. DN Duvick, Nature Reviews Genetics 2:69-73, 2001.
The scientific roots of modern plant biotechnology. IM Sussex, The Plant Cell 20:1189-1198, 2008.
Agrobacterium: nature's genetic engineer. EW Nester, Frontiers in Plant Science 5:1-16, 2015.
Lecture 2. Genetics and phenotype
(Click to see slide show)
(i) Gene design
(ii) Single gene traits
(iii) Reporter genes
(iv) Visualising cell architecture
(Download Lecture 2 notes in PDF form)
Suggested reading
Towards two decades of plant biotechnology: successes, failures and prospects. N Halford Food and Energy Security 1:9-28, 2012.
GM plants: questions and answers. Royal Society Report, 2016
Using intrinsically fluorescent proteins for plant cell imaging. R Dixit, R Cyr and S Gilroy, The Plant Journal 45:599-615, 2006.
Lecture 3. Plant biotechnology
(Click to see slide show)
(i) Genome scale DNA engineering and precision breeding
(ii) Synthetic Biology
(iii) Global challenges
(iv) Problems and technical opportunities.
(Download Lecture 2 notes in PDF form)

Following lectures: CO2 levels, photosynthesis and carbon capture (Hibberd); Nutrient availability (Davies); Global warming: Drought and water relations (Griffiths); Temperature responses (Tanner)
Part 1B Practical Class
Plant transformation and use of reporter genes and microscopy of plants.
In this practical session there are several objectives: (i) to describe gene fusions, (ii) the use of reporter genes in plants, and (iii) the use of microscopy techniques for reporter gene detection in the context of plant transformation.
These pages contain copies of images used in the practical, and articles for further reading are provided as downloadable PDFs (Click on the menu items to access these).